Aqueous Two-Phase Extraction Advances for Bioseparation

Aqueous Two-Phase Extraction Advances for Bioseparation

Aqueous two-phase extraction (ATPE), unique liquid-liquid extraction, involves a transfer of solute from one aqueous phase to another. ATPE includes polymer–polymer type and polymer–salt type systems for the recovery of proteins. The protein must be recovered in a highly purified form in order to improve its quality, decrease energy consumption, reduce waste and minimize costs. To acquire the h...

Aqueous Two-phase Extraction of Invertase from Baker’s Yeast

Extraction of Cephalexin Using Aqueous Two-Phase Systems Composed of Cholinium Chloride and K3PO4

Cephalexin is an important antibiotic. It is very significant to determine an appropriate method for the extraction of this valuable antibiotic for industrial applications. Aiming at developing an efficient method for the extraction of cephalexin, the partitioning of cephalexin has been evaluated in aqueous two-phase system, including cholinium chloride and potassium phosphate. The effect of th...

Aqueous Two-Phase Systems

ger G and Albertsson PA (1983) AfRnity partitioning of phosphofructokinase from baker’s yeast using polymer-bound Cibacron blue F3GA. European Journal of Biochemistry 131: 589}594. KopperschlaK ger G and Birkenmeier G (1990) AfRnity partitioning and extraction of proteins. Bioseparation 1: 235}254. Tjerneld F, Johansson G and Joelsson M (1987) AfRnity liquid}liquid extraction of lactate dehydro...

Aqueous two-phase extraction using thermoseparating polymer: a new system for the separation of endo-polygalacturonase

Aqueous two-phase partitioning of endo-polygalacturonase (endo-PG) produced by Kluyveromyces marxianus strains was studied, on systems containing the thermoseparating polymer Ucon 50-HB-5100 (a random copolymer of 50% ethylene oxide (EO) and 50% propylene oxide (PO)) as one of the phase-forming compounds. Ucon 50-HB-5100 (Ucon)–polyvinyl alcohol (PVA 10,000), Ucon 50-HB-5100–hydroxypropyl starc...